Journal: Heliyon

Article Title: Characterization of primary human leptomeningeal cells in 2D culture

doi: 10.1016/j.heliyon.2024.e26744

Figure Lengend Snippet: Characterisation of the primary human leptomeningeal cells. Leptomeningeal cells stained for vimentin, cytokeratin and desmoplakin (green) and DAPI (blue). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: LMCs were stained for Vimentin [MAB2105, Biotechne], pan-Cytokeratin [AB7753, Abcam], Desmoplakin I + II [sc-390975, Santa-Cruz], CRABP2 [NB19051-7h12, Biotechne], S100A6 [NB100-2590, Biotechne], E-cadherin [SAB4503751, Sigma], Connexin-43 [AB11370, Abcam] and Occludin [SAB3500301, Sigma].

Techniques: Staining

Journal: Molecular Medicine Reports

Article Title: Analysis of different components in the peritumoral tissue microenvironment of colorectal cancer: A potential prospect in tumorigenesis

doi: 10.3892/mmr.2016.5584

Figure Lengend Snippet: Expression levels of CD1, CD133, CK18, α-SMA and vimentin indicated by immunohistochemical staining. Expression levels of (A) CD1, (B) CD133, (C) CK18, (D) α-SMA and (E) vimentin, in samples no. 1, 2 and 3 (left to right). CD1, cyclin D1; CD133, cluster of differentiation 133; CK18, cytokeratin 18; α-SMA, α-smooth muscle actin.

Article Snippet: The primary antibodies used in the IHC staining in the present study were as follows: Rabbit polyclonal against E-cadherin (Abcam, Cambridge, MA, USA; cat. no. ab15148), rat monoclonal against CRB3 (Abcam; cat. no. ab180835), rabbit monoclonal against α-SMA (Abcam; cat. no. 124964), rabbit polyclonal against PAR-3 (Bioss, Inc., Woburn, MA, USA; cat. no. bs-9510R), rabbit polyclonal against Hyal-1 (Abcam; cat. no. ab103977), mouse monoclonal against Col-I (Abcam; cat. no. ab6308), rabbit polyclonal against cytokeratin 18 (CK18; Bioss, Inc.; cat. no. bs-1339R), rabbit polyclonal against vimentin (Bioss, Inc.; cat. no. bs-8533R), rabbit polyclonal against CD1 (Bioss, Inc.; cat. no. bs-0623R) and rabbit polyclonal against CD133 (Bioss, Inc.; cat. no. bs-0395R).

Techniques: Expressing, Immunohistochemical staining, Staining

Journal: Molecular Medicine Reports

Article Title: Analysis of different components in the peritumoral tissue microenvironment of colorectal cancer: A potential prospect in tumorigenesis

doi: 10.3892/mmr.2016.5584

Figure Lengend Snippet: CCA value of ten biomarkers in samples No. 1, No. 2 and No. 3. Demonstrated by the CCA value, the expression levels of E-cadherin, CK18, Crumbs-3 and Par-3 in sample No. 3 were lower compared with sample No. 1 (P<0.05). The expression of CK18 in sample No. 2 was lower than in sample No. 3 (P<0.0001). The expression levels of Hyal-1, Col-I, vimentin, CD1, CD133 and α-SMA in sample No. 3 were higher than in sample No. 1 (P<0.05), however, the expression levels of Hyal-1, Col-I, α-SMA in sample No. 3 were lower than that in sample No. 2, the result for Hyal-1 was not significant. CCA, corrected absorbance value; CK18, cytokeratin 18; Par-3, proteinase activated receptor 3; Hyal-1, hyaluronidase 1; Col-I, collagen type I; CD1, cyclin D1; CD133, cluster of differentiation 133; α-SMA, α-smooth muscle actin.

Article Snippet: The primary antibodies used in the IHC staining in the present study were as follows: Rabbit polyclonal against E-cadherin (Abcam, Cambridge, MA, USA; cat. no. ab15148), rat monoclonal against CRB3 (Abcam; cat. no. ab180835), rabbit monoclonal against α-SMA (Abcam; cat. no. 124964), rabbit polyclonal against PAR-3 (Bioss, Inc., Woburn, MA, USA; cat. no. bs-9510R), rabbit polyclonal against Hyal-1 (Abcam; cat. no. ab103977), mouse monoclonal against Col-I (Abcam; cat. no. ab6308), rabbit polyclonal against cytokeratin 18 (CK18; Bioss, Inc.; cat. no. bs-1339R), rabbit polyclonal against vimentin (Bioss, Inc.; cat. no. bs-8533R), rabbit polyclonal against CD1 (Bioss, Inc.; cat. no. bs-0623R) and rabbit polyclonal against CD133 (Bioss, Inc.; cat. no. bs-0395R).

Techniques: Expressing

Journal: International journal of biological sciences

Article Title: Contact Cooling-Induced ELOVL4 Enhances Skin Wound Healing by Promoting the Inflammation-to-Proliferation Phase Transition.

doi: 10.7150/ijbs.107871

Figure Lengend Snippet: Figure 1. 20°C treatment promotes skin wound healing. A. Schematic of wound healing rate in different seasons. B. K14 and VIM immunostaining shows 20°C treatment promotes wound healing. Scale bars, 50μm. Statistical of the length of the regenerated epidermis. N ≥ 5, **p < 0.01, ns: no significance. C. Volcano Plot shows different expressed genes in the 20°C-treatment group and control group. D. GO shows skin development pathway enriched in the 20°C-treatment group. E. KEGG shows fatty acid elongation pathway enriched in the 20°C-treatment group. F. iPATH shows fatty acid elongation pathway increased after 20°C treatment.

Article Snippet: The following antibodies were used in the study: K14 (Boster, BM4522), VIM (BEYOTIME, AF0318), ELOVL4 (Abclonal, A3639), PCNA (Elabscience, E-AB-22001), CD31 (Servicebio, GB12063-100), TNFα (BEYOTIME, AF8208), P63 (Zenbio, 381215), IL1β (BEYOTIME, AF7209).

Techniques: Immunostaining, Control

Journal: International journal of biological sciences

Article Title: Contact Cooling-Induced ELOVL4 Enhances Skin Wound Healing by Promoting the Inflammation-to-Proliferation Phase Transition.

doi: 10.7150/ijbs.107871

Figure Lengend Snippet: Figure 3. Elovl4-EPA/DHA promotes wound healing. A. Phase-contrast microscope and statistics show wound healing after long chain fatty acid treatment; Statistics of average wound size. Scale bars, 2 mm. N ≥ 5, ***p < 0.001, **p < 0.01, *p < 0.05. B. K14/PCNA and K14/CD31 immunostaining shows wound healing after DHA, EPA, or ELOVL4 inhibitor treatment; Statistics of average PCNA+ and CD31+ cells. Scale bars, 200μm. N ≥ 5, **p < 0.01, *p < 0.05. C. Schematic shows wound healing after DHA, EPA, or ELOVL4 inhibitor treatment.

Article Snippet: The following antibodies were used in the study: K14 (Boster, BM4522), VIM (BEYOTIME, AF0318), ELOVL4 (Abclonal, A3639), PCNA (Elabscience, E-AB-22001), CD31 (Servicebio, GB12063-100), TNFα (BEYOTIME, AF8208), P63 (Zenbio, 381215), IL1β (BEYOTIME, AF7209).

Techniques: Microscopy, Immunostaining

Journal: International journal of biological sciences

Article Title: Contact Cooling-Induced ELOVL4 Enhances Skin Wound Healing by Promoting the Inflammation-to-Proliferation Phase Transition.

doi: 10.7150/ijbs.107871

Figure Lengend Snippet: Figure 5. 20°C treatment can inhibit TNFα-mediated inflammatory response. A. FeaturePlots show increased expression of Il1b and Tnfa in PWD3. B. qRT-PCR shows the expression of inflammatory factors decreased in the 20°C group. N ≥ 5, **p < 0.01, ***p < 0.001, ns: no significance. C. TNFα immunostaining shows the decreased expression of TNFα in the 20°C-treatment group; Statistics of average FI of TNFα. Scale bars, 100μm. N ≥ 5, **p < 0.01, *p < 0.05. D. K14/VIM, K14/CD31, K14/PCNA, and P63/VIM immunostaining shows wound healing in the TNFα group and the 20°C treatment +TNFα group. E. Statistics of the average rate of re-epithelialization. Scale bars, 100μm. N ≥ 5, **p < 0.01, *p < 0.05. F. Schematic shows decreased expression of TNFα after 20°C treatment.

Article Snippet: The following antibodies were used in the study: K14 (Boster, BM4522), VIM (BEYOTIME, AF0318), ELOVL4 (Abclonal, A3639), PCNA (Elabscience, E-AB-22001), CD31 (Servicebio, GB12063-100), TNFα (BEYOTIME, AF8208), P63 (Zenbio, 381215), IL1β (BEYOTIME, AF7209).

Techniques: Expressing, Quantitative RT-PCR, Immunostaining

Journal: International journal of biological sciences

Article Title: Contact Cooling-Induced ELOVL4 Enhances Skin Wound Healing by Promoting the Inflammation-to-Proliferation Phase Transition.

doi: 10.7150/ijbs.107871

Figure Lengend Snippet: Figure 6. Elovl4-EPA/DHA promotes skin organoids to regenerate skin. A. VlnPlot shows expression of Elovl4. B. FeaturePlot shows Elovl4 expressed in epidermal cells. C. Immunostaining shows that ELOVL4 is expressed in epidermal cells. D. TNFα/VIM and IL1β/VIM immunostaining shows the decreased expression of inflammatory factors after LCFAs treatment; Statistics of average FI of TNFα- and IL1β-expressing cells. Scale bars, 20μm. N ≥ 5, **p < 0.01, *p < 0.05, ns: no significance. E. Phase-contrast microscope and statistics show wound healing after long chain fatty acid treatment; Statistics of average wound size. N ≥ 5, **p < 0.01, *p < 0.05. F. K14 / PCNA and TNFα immunostaining shows depressed wound healing in the iELOVL4 transplantation group. Statistics of PCNA+ cells and average FI of TNFα. Scale bars, 100μm. N ≥ 5, *p < 0.05.

Article Snippet: The following antibodies were used in the study: K14 (Boster, BM4522), VIM (BEYOTIME, AF0318), ELOVL4 (Abclonal, A3639), PCNA (Elabscience, E-AB-22001), CD31 (Servicebio, GB12063-100), TNFα (BEYOTIME, AF8208), P63 (Zenbio, 381215), IL1β (BEYOTIME, AF7209).

Techniques: Expressing, Immunostaining, Microscopy, Transplantation Assay

Journal: Cell death & disease

Article Title: Litchi procyanidins inhibit colon cancer proliferation and metastasis by triggering gut-lung axis immunotherapy.

doi: 10.1038/s41419-022-05482-5

Figure Lengend Snippet: Fig. 2 LPC inhibits pulmonary metastasis of CT26 colon cancer. a Schematic view of the experimental procedures of CT26 pulmonary metastatic mouse model. b, c Image and corresponding H&E staining of lung tissue. d Percentage change of body weight. e–g Lung weight, the number of lung tumor nodule, and metastasis rate (n = 6 mice). h Protein expression of PCNA, vimentin and E-cadherin in lungs (n = 3 mice). Data were presented as mean ± SEM, *p < 0.05, **p < 0.01.

Article Snippet: The primary antibodies included β-tubulin (1:5000, 250175, ZEN BIO, China), E-cadherin (1:500, PB9561, BOSTER, China), vimentin (1:500, PB9359, BOSTER, China) and PCNA (1:2000, 10205- 2-AP, Proteintech, China).

Techniques: Staining, Expressing